High resolution preparative column chromatographic system for gangliosides using DEAE-Sephadex and a new porus silica, Iatrobeads

Biochim Biophys Acta. 1976 Sep 27;441(3):488-97.

Abstract

A new high-resolution preparative column chromatographic system was developed for efficient and rapid isolation of ganglioside molecular species. The system involved a combination of ion-exchange and adsorption chromatographies using DEAE-Sephadex A-25 and the newly developed, totally porous silica spheres, Iatrobeads. Using this system the brain gangliosides, GM1, GD1a, GD1b and GT1 were obtained in high purity and in milligram amounts, in a relatively short time, by simple procedures. The presence of a number of unidentified molecular species of gangliosides, which are present only in small amounts, was also demonstrated.

MeSH terms

  • Animals
  • Brain Chemistry
  • Cattle
  • Chromatography / methods
  • Chromatography, Gas
  • Chromatography, Ion Exchange / methods
  • Chromatography, Thin Layer
  • Gangliosides / isolation & purification*
  • Silicon Dioxide

Substances

  • Gangliosides
  • Silicon Dioxide