Effect of omeprazole on gene expression in canine gastric parietal cells

Am J Physiol. 1991 Mar;260(3 Pt 1):G434-9. doi: 10.1152/ajpgi.1991.260.3.G434.

Abstract

Stimulation of gastric parietal cells by carbachol induces coordinate expression of the genes for two enzymes involved in the process of acid secretion, H(+)-K(+)-ATPase and carbonic anhydrase II (CA II). The basis of this coordinate expression was examined in experiments using parietal cells that had been pretreated with omeprazole. We observed a twofold increase in the steady-state mRNA levels of both H(+)-K(+)-ATPase and CA II after cells were treated with the inhibitor. The induction of CA II mRNA by carbachol followed the same kinetics in omeprazole-pretreated cells as in those that were not pretreated, suggesting that the induction of CA II gene expression by carbachol was not dependent on activation of the gastric H(+)-K(+)-ATPase. In addition, carbachol stimulation of omeprazole-pretreated cells resulted in an induction of one or more larger mRNA species that hybridized with the H(+)-K(+)-ATPase probe. The observation that carbachol-induced increases in steady-state levels of beta-actin mRNA in parietal cells could be inhibited by omeprazole pretreatment suggests a possible linkage between increased beta-actin gene expression and the process of acid secretion.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / genetics
  • Adenosine Triphosphatases / genetics*
  • Animals
  • Blotting, Northern
  • Carbonic Anhydrases / genetics*
  • DNA Probes
  • Dogs
  • Gene Expression / drug effects
  • H(+)-K(+)-Exchanging ATPase
  • In Vitro Techniques
  • Kinetics
  • Nucleic Acid Hybridization
  • Omeprazole / pharmacology*
  • Parietal Cells, Gastric / drug effects
  • Parietal Cells, Gastric / enzymology*
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics*
  • RNA, Messenger / isolation & purification

Substances

  • Actins
  • DNA Probes
  • RNA, Messenger
  • Adenosine Triphosphatases
  • H(+)-K(+)-Exchanging ATPase
  • Carbonic Anhydrases
  • Omeprazole