Effects of fluoride on DNA damage, S-phase cell-cycle arrest and the expression of NF-kappaB in primary cultured rat hippocampal neurons

Toxicol Lett. 2008 Jun 10;179(1):1-5. doi: 10.1016/j.toxlet.2008.03.002. Epub 2008 Mar 18.

Abstract

The mechanisms underlying the neurotoxicity of fluorosis still remain obscure. To investigate DNA damage, cell-cycle distribution and expression of nuclear factor kappa B (NF-kappaB) induced by fluoride, the primary rat hippocampal neurons were incubated with various concentrations (20mg/l, 40 mg/l, and 80 mg/l) of sodium fluoride for 24 h in vitro. Our results showed that olive tail moments (OTMs) were significantly elevated in all fluoride-treated groups, while significant increases in the percentage of DNA in the tail were, respectively, observed at 40 mg/l and 80 mg/l levels of fluoride. An increase in the proportion of cells in S-phase was observed in response to the treatment of 40 mg/l and 80 mg/l fluoride. Gene expression of NF-kappaB was also enhanced by fluoride treatment in a dose-dependent manner. The results indicated that fluoride could induce S-phase cell-cycle arrest, up-regulation of NF-kappaB and DNA damage in primary rat hippocampal neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Comet Assay
  • DNA / drug effects*
  • DNA Damage*
  • DNA Replication / drug effects
  • Fetus / cytology
  • Gene Expression
  • Hippocampus / drug effects*
  • Hippocampus / metabolism
  • NF-kappa B / genetics
  • NF-kappa B / metabolism*
  • Neurons / drug effects*
  • Neurons / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • S Phase / drug effects*
  • Sodium Fluoride / toxicity*

Substances

  • NF-kappa B
  • Sodium Fluoride
  • DNA