In vivo anti-inflammatory and antinociceptive actions of some Lamium species

J Ethnopharmacol. 2008 Jun 19;118(1):166-72. doi: 10.1016/j.jep.2008.04.001. Epub 2008 Apr 11.


Ethnopharmacological relevance: Several Lamium species have been used to relieve pain in arthritic ailments in Turkish folk medicine.

Aim of the study: To evaluate the anti-inflammatory and antinociceptive activities of different extracts prepared with methanol, dichloromethane, n-butanol and water from the aerial parts of some Lamium species of Turkish origin.

Materials and methods: Lamium eriocephalum subsp. eriocephalum, Lamium garganicum subsp. laevigatum, Lamium garganicum subsp. pulchrum and Lamium purpureum var. purpureum (Lamiaceae); for the anti-inflammatory activity, carrageenan-induced hind paw edema model, PGE(2)-induced hind paw edema model, and 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema model and for the antinociceptive activity p-benzoquinone (PBQ)-induced writhing test in mice were employed.

Results: The n-butanol extracts of Lamium garganicum subsp. laevigatum (LGL-BuOH), Lamium garganicum subsp. pulchrum (LGP-BuOH), and Lamium purpureum var. purpureum (LPP-BuOH) exhibited notable inhibition (16.5-28.9, 14.5-26.9, 12.3-21.5%, resp.) in carrageenan-induced hind paw edema model at doses of 200mg/kg without inducing any gastric damage. The LGL-BuOH (7.1-30.4%) and LGP-BuOH (5.9-24.1%) extracts also displayed potent anti-inflammatory activity against PGE(2)-induced hind paw edema model. LGL-BuOH and LGP-BuOH were also found to exhibit remarkable antinociceptive activity in p-benzoquinone-induced abdominal constriction test at a dose of 200 mg/kg (25.0 and 24.3%, respectively).

Conclusion: The experimental data demonstrated that Lamium garganicum subsp. laevigatum and Lamium garganicum subsp. pulchrum displayed remarkable anti-inflammatory and antinociceptive activities.

MeSH terms

  • Analgesics / administration & dosage*
  • Analgesics / adverse effects
  • Analgesics / isolation & purification
  • Animals
  • Anti-Inflammatory Agents / administration & dosage*
  • Anti-Inflammatory Agents / adverse effects
  • Anti-Inflammatory Agents / isolation & purification
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Inflammation / drug therapy
  • Lamiaceae / chemistry*
  • Male
  • Medicine, Traditional
  • Mice
  • Pain / drug therapy
  • Plant Extracts / administration & dosage*
  • Plant Extracts / adverse effects
  • Turkey


  • Analgesics
  • Anti-Inflammatory Agents
  • Plant Extracts