A new specific DNA endonuclease activity in yeast mitochondria

Mol Gen Genet. 1991 Feb;225(2):340-1. doi: 10.1007/BF00269867.

Abstract

Two group I intron-encoded proteins from the yeast mitochondrial genome have already been shown to have a specific DNA endonuclease activity. This activity mediates intron insertion by cleaving the DNA sequence corresponding to the splice junction of an intronless strain. We have discovered in mitochondrial extracts from the yeast strain 777-3A a new DNA endonuclease activity which cleaves the fused exon A3-exon A4 junction sequence of the CO XI gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • DNA Restriction Enzymes / genetics*
  • DNA Restriction Enzymes / metabolism
  • Deoxyribonucleases, Type II Site-Specific*
  • Endodeoxyribonucleases / genetics*
  • Endodeoxyribonucleases / metabolism
  • Exons
  • Genes, Fungal
  • Introns
  • Mitochondria / enzymology
  • Molecular Sequence Data
  • RNA Splicing
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins

Substances

  • Saccharomyces cerevisiae Proteins
  • Endodeoxyribonucleases
  • AI3 protein, S cerevisiae
  • DNA Restriction Enzymes
  • Deoxyribonucleases, Type II Site-Specific