Epstein Barr virus/complement C3d receptor is an interferon alpha receptor

EMBO J. 1991 Apr;10(4):919-26.

Abstract

Interferon alpha contains a sequence motif similar to the complement receptor type two (CR2/CD21) binding site on complement fragment C3d. Antibodies against a peptide with the CR2 binding sequence on C3d react with a peptide carrying the IFN alpha CR2 binding motif (residues 92-99) and with recombinant IFN alpha. The IFN alpha-derived peptide, as well as recombinant IFN alpha, inhibits C3bi/C3d interaction with CR2 on the Burkitt lymphoma Raji. The direct interaction of IFN alpha and CR2 is inhibited by polyclonal anti-IFN alpha, anti-CR2 and anti-C3d peptide antibodies as well as by C3bi/C3d, EBV coat protein gp350/220 and IFN but not by IFN gamma. [125I]IFN alpha binding to Raji cells is inhibited by polyclonal anti-IFN alpha and anti-CR2 antibodies, by peptides with the CR2 binding motif and partially by C3bi/C3d. Monoclonal anti-CR2 antibody HB5, but not OKB-7, blocks IFN alpha binding to Raji cells. CR2 or CR2-like molecules may therefore be the major IFN alpha receptors on B lymphocytes.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, Differentiation, B-Lymphocyte / genetics
  • Antigens, Differentiation, B-Lymphocyte / metabolism
  • B-Lymphocytes / immunology
  • Cell Line
  • Complement C3d / metabolism
  • Enzyme-Linked Immunosorbent Assay
  • Herpesvirus 4, Human / physiology*
  • Humans
  • Interferon Type I / metabolism
  • L Cells / immunology
  • Lymphocyte Activation
  • Mice
  • Molecular Sequence Data
  • Receptors, Complement / genetics*
  • Receptors, Complement / metabolism
  • Receptors, Complement 3d
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism
  • Receptors, Interferon
  • Receptors, Virus / genetics*
  • Receptors, Virus / physiology
  • Recombinant Proteins
  • Rosette Formation
  • Sequence Homology, Nucleic Acid
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, B-Lymphocyte
  • Interferon Type I
  • Receptors, Complement
  • Receptors, Complement 3d
  • Receptors, Immunologic
  • Receptors, Interferon
  • Receptors, Virus
  • Recombinant Proteins
  • Complement C3d