Development of real-time in vivo imaging of device-related Staphylococcus epidermidis infection in mice and influence of animal immune status on susceptibility to infection

J Infect Dis. 2008 Jul 15;198(2):258-61. doi: 10.1086/589307.

Abstract

Staphylococcus epidermidis is the leading cause of hospital-acquired device-related infections, but there is a lack of suitable methods to investigate the pathogenesis of S. epidermidis infection. We created a bioluminescent strain of S. epidermidis and developed a subcutaneous catheter-related murine infection model for real-time monitoring of biofilm-associated infection. Additionally, we compared severely immunocompromised and immunocompetent mice, demonstrating the substantial effect of animal immune status on susceptibility to experimentally induced S. epidermidis disease. This study presents a novel approach for investigating the in vivo details of the pathogenesis of S. epidermidis infection.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biofilms
  • Colony-Forming Units Assay
  • Disease Susceptibility / immunology*
  • Female
  • Luminescence
  • Mice
  • Mice, SCID
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / immunology*
  • Staphylococcus epidermidis*
  • T-Lymphocytes / immunology
  • T-Lymphocytes, Cytotoxic / immunology