Primary structure and functional expression from complementary DNA of a brain calcium channel

Nature. 1991 Apr 4;350(6317):398-402. doi: 10.1038/350398a0.


The primary structure of a voltage-dependent calcium channel from rabbit brain has been deduced by cloning and sequencing the complementary DNA. Calcium channel activity expressed from the cDNA is dramatically increased by coexpression of the alpha 2 and beta subunits, known to be associated with the dihydropyridine receptor. This channel is a high voltage-activated calcium channel that is insensitive both to nifedipine and to omega-conotoxin. We suggest that it is expressed predominantly in cerebellar Purkinje cells and granule cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Brain
  • Calcium Channels / genetics*
  • Calcium Channels / physiology
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Microinjections
  • Molecular Sequence Data
  • RNA, Messenger / administration & dosage
  • RNA, Messenger / genetics
  • Rabbits
  • Tissue Distribution
  • Xenopus


  • Calcium Channels
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/X57450
  • GENBANK/X57451
  • GENBANK/X57452
  • GENBANK/X57453
  • GENBANK/X57454
  • GENBANK/X57455
  • GENBANK/X57476
  • GENBANK/X57477
  • GENBANK/X57688
  • GENBANK/X57689