Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically

J Proteome Res. 2008 Jul;7(7):3060-3. doi: 10.1021/pr8001029. Epub 2008 May 22.


In-depth human erythrocyte proteome studies are severely hampered by the presence of hemoglobin and carbonic anhydrase-1, which account for more than 98% of the total erythrocyte soluble protein content. We developed a specific depletion approach that resulted in a drastic increase in the number of identified proteins. This depletion technique is valuable for proteome studies of human erythrocyte disorders with unknown etiology and of tissue samples that contain blood.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Proteins / analysis*
  • Carbonic Anhydrase I / blood
  • Carbonic Anhydrase I / isolation & purification
  • Erythrocytes / chemistry*
  • Hemoglobins / isolation & purification
  • Humans
  • Proteome / analysis*
  • Solubility


  • Blood Proteins
  • Hemoglobins
  • Proteome
  • Carbonic Anhydrase I