Effects of surfactant, salt and solvent on the structure and activity of adenosine deaminase: molecular dynamic and spectrophotometric studies

Int J Biol Macromol. 2008 Aug 15;43(2):151-8. doi: 10.1016/j.ijbiomac.2008.04.007. Epub 2008 Apr 16.

Abstract

Effects of sodium dodecyl sulfate, dodecyltrimethylammonium bromide, sodium chloride, sodium sulfate, methanol and ethanol, on the structure and activity of adenosine deaminase (ADA) were investigated by UV-Vis, circular dichroism spectrophotometry and molecular dynamics (MDs) studies. Relative activity, experimental and computational helix content, total accessible surface area (ASA) and exposed charged surface area (ECSA) were obtained. The relative activity of ADA in the absence and the presence of denaturants were compared with structural results. It was shown that an increase in the surface area and a decrease in the amount of helicity are associated with a decrease in the activity of ADA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Deaminase / chemistry*
  • Circular Dichroism
  • Ethanol / chemistry
  • Kinetics
  • Methanol / chemistry
  • Quantitative Structure-Activity Relationship*
  • Quaternary Ammonium Compounds / chemistry
  • Sodium Chloride / chemistry
  • Sodium Dodecyl Sulfate / chemistry
  • Solvents / chemistry*
  • Spectrophotometry, Ultraviolet
  • Sulfates / chemistry
  • Surface-Active Agents / chemistry*

Substances

  • Quaternary Ammonium Compounds
  • Solvents
  • Sulfates
  • Surface-Active Agents
  • sodium sulfate
  • Sodium Dodecyl Sulfate
  • Ethanol
  • Sodium Chloride
  • Adenosine Deaminase
  • dodecyltrimethylammonium
  • Methanol