Abstract
Degenerate oligonucleotide probes encoding sequences conserved among mammalian protein-tyrosine-phosphatases (PTPases) were used to amplify DNA fragments from a Schizosaccharomyces pombe cDNA library by polymerase chain reaction (PCR) methods. A cloned PCR product predicted peptide sequences similar to those found in PTPases but not identical to any published sequences. A S. pombe gene, designated pyp1+, was identified in a cDNA library with this PCR probe, cloned, and sequenced. The sequence of the gene predicted a 550-amino acid protein with Mr 61,586, which includes amino acid sequences that are highly conserved in mammalian PTPases. Disruption of the pyp1+ gene resulted in viable cells. Overexpression of the pyp1+ gene in S. pombe permitted detection of a protein of apparent Mr 63,000.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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DNA Mutational Analysis
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DNA, Fungal / genetics
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Gene Expression
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Genes, Fungal
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Molecular Sequence Data
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Oligonucleotides / chemistry
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Phosphoprotein Phosphatases / genetics*
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Phosphoprotein Phosphatases / immunology
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Polymerase Chain Reaction
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Precipitin Tests
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Protein Tyrosine Phosphatases
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RNA, Messenger / genetics
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Schizosaccharomyces / genetics*
Substances
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DNA, Fungal
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Oligonucleotides
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RNA, Messenger
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Phosphoprotein Phosphatases
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Protein Tyrosine Phosphatases
Associated data
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GENBANK/M60366
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GENBANK/M60367
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GENBANK/M60368
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GENBANK/M60369
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GENBANK/M62382
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GENBANK/M62383
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GENBANK/M62384
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GENBANK/M62385
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GENBANK/M62386
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GENBANK/M63257