Numerous transitions in human parainfluenza virus 3 RNA recovered from persistently infected cells

Virology. 1991 Apr;181(2):760-3. doi: 10.1016/0042-6822(91)90913-v.


The nucleotide sequences at the 3'-termini of human parainfluenza virus 3 (HPIV3) genomic RNAs recovered from two lines of persistently infected LLC-MK2 cells were determined following PCR amplification. After 29 months of persistence the 3'-end of the HPIV3 genome was found to be highly mutated. Interestingly, the only types of nucleotide changes observed were U to C and A to G transitions. Both U to C and A to G transitions were present on individual RNA molecules. The data indicate that biased hypermutational activity leading to U To C and A to G mutations operates in cultured cells during persistent HPIV3 infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line
  • Genes, Viral
  • Humans
  • Molecular Sequence Data
  • Mutation
  • Paramyxoviridae Infections / genetics
  • Polymerase Chain Reaction
  • RNA, Viral / chemistry*
  • Respirovirus / genetics*


  • RNA, Viral

Associated data

  • GENBANK/M59049
  • GENBANK/M59050
  • GENBANK/M64453
  • GENBANK/M64454
  • GENBANK/M64458
  • GENBANK/M64461
  • GENBANK/M64464
  • GENBANK/M64467
  • GENBANK/M64469
  • GENBANK/M64470