Changes of the hepatic proteome in hepatitis B-infected mouse model at early stages of fibrosis

J Proteome Res. 2008 Jul;7(7):2642-53. doi: 10.1021/pr7006522. Epub 2008 May 22.

Abstract

Liver fibrosis (LF) is the accumulation of extracellular matrix (ECM) proteins due to chronic liver injury. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to perform a comparative analysis of cytosolic and nuclear protein patterns of nontransgenic (NTg) and HBV transgenic (Tg) mice livers at early stages of fibrosis. We identified several candidate proteins, involved in a variety of pathways, which could be used as putative biomarkers for LF early detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Cell Nucleus / metabolism
  • Cytosol / metabolism
  • Electrophoresis, Gel, Two-Dimensional
  • Hepatitis B / complications*
  • Keratins / metabolism
  • Liver / metabolism*
  • Liver / pathology
  • Liver Cirrhosis / etiology
  • Liver Cirrhosis / genetics
  • Liver Cirrhosis / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Nuclear Proteins / metabolism
  • Phosphorylation
  • Proteome / metabolism*
  • Superoxide Dismutase / biosynthesis

Substances

  • Biomarkers
  • Nuclear Proteins
  • Proteome
  • Keratins
  • Superoxide Dismutase