Production of dimer-specific and dengue virus group cross-reactive mouse monoclonal antibodies to the dengue 2 virus non-structural glycoprotein NS1

J Gen Virol. 1991 Apr;72 ( Pt 4):961-5. doi: 10.1099/0022-1317-72-4-961.

Abstract

A panel of mouse monoclonal antibodies (MAbs) raised against the non-structural glycoprotein NS1 of dengue 2 virus (PR159) was studied for cross-reactivity with the NS1 protein of other dengue virus serotypes and other members of the Flaviviridae using immunoblotting. Most of the 35 anti-NS1 MAbs were found to be specific for dengue 2 virus NS1 (some of which were specific for the native, dimeric form of this protein), but others were found to cross-react within the dengue virus group. This latter group of MAbs, although dominated by MAbs defining a dengue 2 and 4 virus subgroup, also contained some MAbs that were shown to cross-react with both linear (sequential) and conformational epitopes common to the NS1 glycoproteins of all four dengue virus serotypes. Several of these MAbs were also able to cross-react with other flaviviruses, most notably viruses from the Japanese encephalitis antigenic complex. Although cross-reactive epitopes were previously demonstrated on this glycoprotein using polyclonal sera from dengue virus-infected animals and human, this is the first report of the isolation of MAbs which define these determinants and which will allow their further analysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Antigens, Viral / immunology
  • Capsid / analysis
  • Capsid / immunology*
  • Cell Line
  • Cross Reactions
  • Dengue Virus / immunology*
  • Flavivirus / immunology*
  • Immunoblotting
  • Macromolecular Substances
  • Mice
  • Mice, Inbred BALB C / immunology
  • Vero Cells
  • Viral Core Proteins / analysis
  • Viral Core Proteins / immunology*
  • Viral Nonstructural Proteins

Substances

  • Antibodies, Monoclonal
  • Antigens, Viral
  • Macromolecular Substances
  • Viral Core Proteins
  • Viral Nonstructural Proteins