Determination of phenolic compounds in Yucca gloriosa bark and root by LC-MS/MS

J Pharm Biomed Anal. 2008 Aug 5;47(4-5):854-9. doi: 10.1016/j.jpba.2008.04.011. Epub 2008 May 23.


On the basis of the biological activities shown by yuccaols and gloriosaols from Yucca schidigera and Yucca gloriosa, the content of yuccaols and gloriosaols in two different parts of Y. gloriosa (roots and bark), was determined for each single compound, and compared with phenolic determination in Y. schidigera bark, concluding that Y. gloriosa bark and roots are rich sources of phenolic derivatives structurally related to resveratrol. LC/ESIMS (liquid chromatography coupled to electrospray mass spectrometry) qualitative and an LC/ESIMS/MS (liquid chromatography coupled to tandem electrospray mass spectrometry) quantitative studies of the phenolic fraction of Y. gloriosa were performed. LC/ESIMS/MS multiple reaction monitoring (MRM) method previously described for yuccaols in Y. schidigera was applied and optimised for separation and determination of gloriosaols and yuccaols in Y. gloriosa. Due to the sensitivity and the repeatability of the assay, we suggest this method as suitable for industrial quality control of raw materials and final products.

MeSH terms

  • Calibration
  • Chromatography, Liquid / methods*
  • Methanol / chemistry
  • Phenols / analysis*
  • Phenols / chemistry
  • Phenols / isolation & purification
  • Plant Bark / chemistry*
  • Plant Extracts / chemistry
  • Plant Roots / chemistry*
  • Powders / chemistry
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization
  • Tandem Mass Spectrometry / methods*
  • Temperature
  • Time Factors
  • Ultrasonics
  • Volatilization
  • Yucca / chemistry*


  • Phenols
  • Plant Extracts
  • Powders
  • Methanol