Abstract
Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H(2)O(2)-producing lactobacilli were not associated with protection against VVC.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Analysis of Variance
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Brazil / epidemiology
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Candidiasis, Vulvovaginal / microbiology
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Chi-Square Distribution
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DNA, Bacterial / isolation & purification
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DNA, Ribosomal Spacer / isolation & purification
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Electrophoresis, Gel, Pulsed-Field
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Female
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Humans
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Hydrogen Peroxide / metabolism
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Lactobacillus / classification
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Lactobacillus / isolation & purification*
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Lactobacillus / metabolism
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Polymerase Chain Reaction
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Vagina / microbiology*
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Vaginal Discharge / microbiology*
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Vaginosis, Bacterial / epidemiology
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Vaginosis, Bacterial / microbiology*
Substances
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DNA, Bacterial
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DNA, Ribosomal Spacer
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Hydrogen Peroxide