[Investigation of ochratoxin a, B and citrinin contamination in various commercial foods]

Shokuhin Eiseigaku Zasshi. 2008 Apr;49(2):111-5. doi: 10.3358/shokueishi.49.111.
[Article in Japanese]

Abstract

Ochratoxin A (OTA), ochratoxin B (OTB) and citrinin (CIT) in commercial foods were simultaneously determined and confirmed with high-performance liquid chromatography (HPLC) and liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). The samples examined were made up of cereal, fruit, coffee, and cacao products. The limits of quantification (S/N> or =10) of OTA, OTB and CIT were 0.1 microg/kg or less. Aflatoxins (AF), deoxynivalenol (DON) and fumonisins were also surveyed. Of 157 samples examined, 44 were contaminated with OTA at levels of 0.11 to 4.0 microg/kg. At least 2 positive samples were labeled as domestics. In most positive samples, the OTA level was low, less than 1 microg/kg. The highest incidence of OTA was observed in cacao powder (10/12), followed by instant coffee (5/7), cocoa (5/8) and raisin (6/13). OTB was found in fruit and cacao products containing relatively high levels of OTA. Co-occurrence of OTA, CIT and DON was found in cereal products, and co-occurrence of OTA and AF was found in cacao products. Approximately 30% of naturally contaminated OTA in roasted coffee bean moved into the extract solution when brewed with paper filter.

Publication types

  • English Abstract

MeSH terms

  • Aflatoxins / analysis
  • Cacao / chemistry
  • Chromatography, High Pressure Liquid
  • Chromatography, Liquid
  • Citrinin / analysis*
  • Coffee / chemistry
  • Edible Grain / chemistry
  • Food Analysis / methods*
  • Food Contamination / analysis*
  • Fruit / chemistry
  • Fumonisins / analysis
  • Ochratoxins / analysis*
  • Tandem Mass Spectrometry
  • Trichothecenes / analysis

Substances

  • Aflatoxins
  • Coffee
  • Fumonisins
  • Ochratoxins
  • Trichothecenes
  • ochratoxin A
  • Citrinin
  • ochratoxin B
  • deoxynivalenol