Analysis of iridoids from Harpagophytum and eleutherosides from Eleutherococcus senticosus in horse urine

Biomed Chromatogr. 2008 Aug;22(8):912-7. doi: 10.1002/bmc.1030.


LC/ESI-MS n methods have been previously set up to detect the administration of (i) Harpagophytum and (ii) preparations containing a plant capable of anti-stress properties: Eleutherococcus senticosus. Harpagoside has been found to be the main indicator of Harpagophytum administration in the horse. These methods have been applied to a large number of horse urine samples of various origins. Regarding the detection of Harpagophytum administration, harpagoside, harpagide and 8-para-coumaroyl harpagide were detected together in only one sample out of 317. Eleutheroside E was found to be the main indicator of Eleutherococcus senticosus administration. It was detected in post-administration samples collected from two horses having received a feed supplement containing Eleutherococcus senticosus for several days. Out of the 382 samples tested, eleutheroside E was found in an unexpected large number of urine samples (39%) of various origins and its presence cannot be only due to the sole use of herbal dietary supplements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Liquid / methods
  • Eleutherococcus / chemistry*
  • Horses
  • Iridoids / urine*
  • Mass Spectrometry / methods
  • Pedaliaceae / chemistry*
  • Plant Extracts / urine*


  • Iridoids
  • Plant Extracts
  • eleutherosides