Tn5-rpsL: a new derivative of transposon Tn5 useful in plasmid curing

Gene. 1991 Mar 1;99(1):101-4. doi: 10.1016/0378-1119(91)90039-e.

Abstract

The rpsL gene of Escherichia coli was inserted into the BamHI site of transposon Tn5. This transposon was called Tn5-rpsL. Tn5-rpsL may be useful in microbiological studies when one wants to cure various bacterial genera of certain plasmid(s). A streptomycin-resistant (SmR) derivative of the host bacterial strain is first isolated. The plasmid(s) later to be cured are then labelled with Tn5-rpsL, which makes the cells Sm-sensitive. These cells can regain their resistance to Sm if they lose the Tn5-rpsL-tagged plasmid. Thus, plasmid-free bacteria are easily selected among SmR survivors. The frequency of occurrence of the plasmid-less variants of plasmid-containing wild-type Salmonella typhimurium measured by this method is given as an example.

MeSH terms

  • DNA Transposable Elements*
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Genetic Engineering / methods
  • Plasmids*
  • Restriction Mapping
  • Salmonella typhimurium / genetics

Substances

  • DNA Transposable Elements