Supplementation with orange and blackcurrant juice, but not vitamin E, improves inflammatory markers in patients with peripheral arterial disease

Br J Nutr. 2009 Jan;101(2):263-9. doi: 10.1017/S0007114508995660. Epub 2008 May 28.


Inflammation and endothelial activation are associated with an increased risk of CVD and epidemiological evidence suggests an association between levels of markers of inflammation or endothelial activation and the intake of fruit. Also, vitamin E, a fat-soluble antioxidant, has anti-inflammatory properties. We performed a randomised 2 x 2 factorial, crossover trial to determine the effect of orange and blackcurrant juice (500 ml/d) and vitamin E (15 mg RRR-alpha-tocopherol/d) supplementation on markers of inflammation and endothelial activation in forty-eight patients with peripheral arterial disease. Patients were randomly allocated to two dietary supplements from the four possible combinations of juice and vitamin E: juice+vitamin E; juice+placebo; reference beverage (sugar drink)+vitamin E; and reference beverage+placebo. The supplementations were given for 28 d, separated by a 4-week wash-out period. Analysis of main effects showed that juice decreased C-reactive protein (CRP) by 11% and fibrinogen by 3% while the reference drink increased CRP by 13% and fibrinogen by 2% (P<0.008 and P<0.002, respectively). No significant differences were measured for IL-6 and the endothelial activation markers von Willebrand factor, tissue-plasminogen activator and plasmin activator inhibitor-1. Vitamin E supplementation had no significant effects on the various markers. We observed no significant interaction between juice and vitamin E. In this study, orange and blackcurrant juice reduced markers of inflammation, but not markers of endothelial activation, in patients with peripheral arterial disease, relative to sugar drinks.

Publication types

  • Randomized Controlled Trial
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / administration & dosage*
  • Beverages*
  • Biomarkers / blood
  • C-Reactive Protein / analysis
  • Citrus sinensis*
  • Dietary Supplements
  • Female
  • Fibrinogen / analysis
  • Humans
  • Interleukin-6 / blood
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Oxidative Stress
  • Peripheral Vascular Diseases / blood
  • Peripheral Vascular Diseases / diet therapy*
  • Ribes*
  • Statistics, Nonparametric
  • Vitamin E / administration & dosage*
  • von Willebrand Factor / analysis


  • Antioxidants
  • Biomarkers
  • Interleukin-6
  • von Willebrand Factor
  • Vitamin E
  • Fibrinogen
  • C-Reactive Protein