Ischemic preconditioning produces systemic protective and adoptively transferable effects

Kidney Int. 2008 Sep;74(5):622-30. doi: 10.1038/ki.2008.208. Epub 2008 May 28.


Ischemic preconditioning directly protects organs from subsequent non-specific injuries. To test for systemic protective effects kidneys from F-344 donor rats went through a short warm ischemic time. Both, clamped and contralateral unclamped kidneys were procured after either a short (15 min) or long (24 h) reperfusion period and transplanted into Lewis rats following a prolonged cold ischemia. To test for transferable effects serum from preconditioned rats was infused either into native donors or recipients. Following a short reperfusion interval protective effects were only evident in previously clamped grafts. However, after a long reperfusion interval protective effects were observed in previously clamped and contralateral unclamped kidneys promoting improved survival, structure, function and reduced inflammation. These effects were not related to heme oxygenase-1 induction or neural transmission as heme oxygenase-1 inhibition or denervation prior to preconditioning did not affect organ protection. These results show that renal ischemic preconditioning is associated with time-dependent local and systemically transferable protection.

MeSH terms

  • Animals
  • Blood Transfusion
  • Enzyme Inhibitors / pharmacology
  • Graft Survival
  • Heme Oxygenase (Decyclizing) / antagonists & inhibitors
  • Ischemic Preconditioning / methods*
  • Kidney / blood supply*
  • Kidney / injuries
  • Kidney / innervation
  • Kidney Transplantation / adverse effects
  • Kidney Transplantation / methods*
  • Kidney Transplantation / pathology
  • Protoporphyrins / pharmacology
  • Rats
  • Rats, Inbred F344
  • Rats, Inbred Lew
  • Reperfusion Injury / pathology
  • Reperfusion Injury / physiopathology
  • Reperfusion Injury / prevention & control*
  • Synaptic Transmission
  • Time Factors


  • Enzyme Inhibitors
  • Protoporphyrins
  • zinc protoporphyrin
  • Heme Oxygenase (Decyclizing)
  • Hmox1 protein, rat