Although the measurement of eye pupil variations is a common method in the only few cannabis effect research, there are no studies on short term effects of kif (Moroccan traditional preparation of cannabis) on eye pupil. The aim of the present paper is to present results about effect of a smoked kif preparation (Cannabis sativa L.) on pupil diameter variations after 30 mn. Two examiners measured the pupil diameter variations before and after kif smoking in 34 eyes of 17 volunteer-consumers in a dark closed room. Pupil diameter was estimated by Colvard pupillometer. Results reveal a significantly increase in pupil size post kif.