Abstract
Neutrophils activated by the formyl peptide f-Met-Leu-Phe transiently accumulate a small subset of highly polar inositol lipids. A similar family of lipids also appear in many other cells in response to a range of growth factors and activated oncogenes, and are presumed to be the direct or indirect products of 3-phosphatidylinositol kinase. The structures of these lipids are shown to be phosphatidylinositol 3-phosphate, phosphatidylinositol-(3,4)bisphosphate and phosphatidylinositol-(3,4,5)trisphosphate, and we present evidence that in intact neutrophils a phosphatidyl-inositol-(4,5)bisphosphate-3-kinase seems to be the focal point through which agonists stimulate the formation of 3-phosphorylated inositol lipids.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Humans
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In Vitro Techniques
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Inositol 1,4,5-Trisphosphate / biosynthesis
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Inositol 1,4,5-Trisphosphate / blood*
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Inositol 1,4,5-Trisphosphate / isolation & purification
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Kinetics
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Neutrophils / drug effects
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Neutrophils / physiology*
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Phosphates / blood
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Phosphatidylinositol Phosphates
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Phosphatidylinositols / biosynthesis
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Phosphatidylinositols / blood*
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Phosphatidylinositols / isolation & purification
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Phosphorus Radioisotopes
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Radioisotope Dilution Technique
Substances
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Phosphates
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Phosphatidylinositol Phosphates
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Phosphatidylinositols
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Phosphorus Radioisotopes
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N-Formylmethionine Leucyl-Phenylalanine
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Inositol 1,4,5-Trisphosphate