Pathway of phosphatidylinositol(3,4,5)-trisphosphate synthesis in activated neutrophils

Nature. 1991 May 2;351(6321):33-9. doi: 10.1038/351033a0.


Neutrophils activated by the formyl peptide f-Met-Leu-Phe transiently accumulate a small subset of highly polar inositol lipids. A similar family of lipids also appear in many other cells in response to a range of growth factors and activated oncogenes, and are presumed to be the direct or indirect products of 3-phosphatidylinositol kinase. The structures of these lipids are shown to be phosphatidylinositol 3-phosphate, phosphatidylinositol-(3,4)bisphosphate and phosphatidylinositol-(3,4,5)trisphosphate, and we present evidence that in intact neutrophils a phosphatidyl-inositol-(4,5)bisphosphate-3-kinase seems to be the focal point through which agonists stimulate the formation of 3-phosphorylated inositol lipids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • In Vitro Techniques
  • Inositol 1,4,5-Trisphosphate / biosynthesis
  • Inositol 1,4,5-Trisphosphate / blood*
  • Inositol 1,4,5-Trisphosphate / isolation & purification
  • Kinetics
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • Phosphates / blood
  • Phosphatidylinositol Phosphates
  • Phosphatidylinositols / biosynthesis
  • Phosphatidylinositols / blood*
  • Phosphatidylinositols / isolation & purification
  • Phosphorus Radioisotopes
  • Radioisotope Dilution Technique


  • Phosphates
  • Phosphatidylinositol Phosphates
  • Phosphatidylinositols
  • Phosphorus Radioisotopes
  • N-Formylmethionine Leucyl-Phenylalanine
  • Inositol 1,4,5-Trisphosphate