Beta1 integrins regulate chondrogenesis and rock signaling in adipose stem cells

Biochem Biophys Res Commun. 2008 Aug 8;372(4):547-52. doi: 10.1016/j.bbrc.2008.05.063. Epub 2008 Jun 2.


Beta1 integrins play a controversial role during chondrogenesis. Since the maturation of chondrocytes relies on a signaling switch from cell-cell to cell-matrix interactions, we hypothesized that beta1 integrins play a different role at the earlier (mainly cell-cell interaction) from the later stage (mainly cell-matrix interaction) of chondrogenesis. Our data showed: in plain medium, sox9, collagen X, and collagen II gene expressions of ASCs were induced by beta1-integrin blockage at day 14. In chondrogenic medium, however, sox 9, sox6, and collagen II gene expression were induced at day 4 but inhibited at day 14. In addition, both beta1-integrin blockage and TGF-beta1 down-regulated Rock-1 and -2 gene expression and produced the round cells. We concluded that beta1 integrins play a more important role at the later stages than earlier stages of chondrogenesis, and that the onset of chondrogenesis promoted by beta1-integrin blockage might be through inhibiting Rock signaling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology*
  • Adipose Tissue / drug effects
  • Cell Shape / genetics
  • Chondrogenesis* / genetics
  • Collagen Type II / genetics
  • Collagen Type X / genetics
  • Culture Media
  • DNA-Binding Proteins / genetics
  • Gene Expression / drug effects
  • Gene Expression Regulation, Developmental*
  • High Mobility Group Proteins / genetics
  • Humans
  • Immunoglobulin G / pharmacology
  • Integrin beta1 / pharmacology
  • Integrin beta1 / physiology*
  • SOX9 Transcription Factor
  • SOXD Transcription Factors
  • Stem Cells / cytology*
  • Stem Cells / drug effects
  • Transcription Factors / genetics
  • Transforming Growth Factor beta1 / antagonists & inhibitors
  • Transforming Growth Factor beta1 / pharmacology
  • Transforming Growth Factor beta1 / physiology
  • rho-Associated Kinases / genetics*


  • Collagen Type II
  • Collagen Type X
  • Culture Media
  • DNA-Binding Proteins
  • High Mobility Group Proteins
  • Immunoglobulin G
  • Integrin beta1
  • SOX6 protein, human
  • SOX9 Transcription Factor
  • SOX9 protein, human
  • SOXD Transcription Factors
  • Transcription Factors
  • Transforming Growth Factor beta1
  • rho-Associated Kinases