Transcriptional regulation of ketone body-utilizing enzyme, acetoacetyl-CoA synthetase, by C/EBPalpha during adipocyte differentiation

Biochim Biophys Acta. 2008 Jun-Jul;1779(6-7):414-9. doi: 10.1016/j.bbagrm.2008.05.001. Epub 2008 May 9.

Abstract

Acetoacetyl-CoA synthetase (AACS), an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies, was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity in 3T3-L1 adipocytes. Moreover, we found that AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Acetate-CoA Ligase / genetics*
  • Adipocytes / cytology*
  • Adipocytes / enzymology
  • Adipocytes / metabolism*
  • Adipogenesis / genetics
  • Adipogenesis / physiology
  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • CCAAT-Enhancer-Binding Protein-alpha / metabolism*
  • Cell Differentiation
  • Chromatin Immunoprecipitation
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Electrophoretic Mobility Shift Assay
  • Gene Expression Profiling
  • Ketone Bodies / metabolism*
  • Male
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Transcription, Genetic

Substances

  • CCAAT-Enhancer-Binding Protein-alpha
  • DNA Primers
  • DNA, Complementary
  • Ketone Bodies
  • RNA, Messenger
  • Acetate-CoA Ligase