Abstract
1. Using indo-1 as a calcium fluorescent probe, we have observed the following in striatal astrocytes in primary culture. 2. The stimulation of alpha-adrenoceptors induces a rapid rise in cytosolic calcium resulting from an internal calcium mobilization followed by an external calcium influx (4-min duration). 3. The stimulation of beta 1-adrenoceptors evokes only a slight internal calcium mobilization (90-sec duration). 4. The simultaneous stimulation of beta 1- and alpha 1-adrenoceptors induces a more prolonged calcium influx (10 min). The latter phenomenon could explain the calcium-dependent synergistic effects of alpha 1 and beta stimulation on cAMP production already described in the brain.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Albuterol / pharmacology
-
Animals
-
Astrocytes / drug effects
-
Astrocytes / metabolism*
-
Calcium / metabolism*
-
Cells, Cultured
-
Corpus Striatum / cytology*
-
Corpus Striatum / drug effects
-
Drug Synergism
-
Ion Channel Gating / drug effects*
-
Isoproterenol / pharmacology
-
Methoxamine / pharmacology
-
Mice
-
Norepinephrine / pharmacology
-
Prazosin / pharmacology
-
Propranolol / pharmacology
-
Receptors, Adrenergic, alpha / drug effects
-
Receptors, Adrenergic, alpha / physiology*
-
Receptors, Adrenergic, beta / drug effects
-
Receptors, Adrenergic, beta / physiology*
-
Yohimbine / pharmacology
Substances
-
Receptors, Adrenergic, alpha
-
Receptors, Adrenergic, beta
-
Yohimbine
-
Propranolol
-
Methoxamine
-
Isoproterenol
-
Albuterol
-
Calcium
-
Norepinephrine
-
Prazosin