The p53 target protein Wig-1 binds hnRNP A2/B1 and RNA Helicase A via RNA

FEBS Lett. 2008 Jun 25;582(15):2173-7. doi: 10.1016/j.febslet.2008.04.065. Epub 2008 Jun 2.

Abstract

The p53-induced Wig-1 gene encodes a double stranded RNA-binding zinc finger protein. We generated Saos-2 osteosarcoma cells expressing tetracycline-inducible Flag-tagged human Wig-1. Induction of Wig-1 expression by doxycycline inhibited cell growth in a long-term assay but did not cause any changes in cell cycle distribution nor increased fraction of apoptotic cells. Using co-immunoprecipitation and mass spectrometry, we identified two Wig-1-binding proteins, hnRNP A2/B1 and RNA Helicase A, both of which are involved in RNA processing. The binding was dependent on the presence of RNA. Our results establish a link between the p53 tumor suppressor and RNA processing via hnRNPA2/B1 and RNA Helicase A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • DEAD-box RNA Helicases / metabolism*
  • Heterogeneous-Nuclear Ribonucleoprotein Group A-B / metabolism*
  • Humans
  • Immunoprecipitation
  • Neoplasm Proteins / metabolism*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Point Mutation
  • RNA / metabolism*
  • RNA Processing, Post-Transcriptional
  • RNA-Binding Proteins
  • Tetracycline / pharmacology
  • Tumor Suppressor Protein p53 / metabolism*
  • Zinc Fingers

Substances

  • Carrier Proteins
  • Heterogeneous-Nuclear Ribonucleoprotein Group A-B
  • Neoplasm Proteins
  • Nuclear Proteins
  • RNA-Binding Proteins
  • Tumor Suppressor Protein p53
  • ZMAT3 protein, human
  • hnRNP A2
  • RNA
  • DHX9 protein, human
  • DEAD-box RNA Helicases
  • Tetracycline