Abstract
The p53-induced Wig-1 gene encodes a double stranded RNA-binding zinc finger protein. We generated Saos-2 osteosarcoma cells expressing tetracycline-inducible Flag-tagged human Wig-1. Induction of Wig-1 expression by doxycycline inhibited cell growth in a long-term assay but did not cause any changes in cell cycle distribution nor increased fraction of apoptotic cells. Using co-immunoprecipitation and mass spectrometry, we identified two Wig-1-binding proteins, hnRNP A2/B1 and RNA Helicase A, both of which are involved in RNA processing. The binding was dependent on the presence of RNA. Our results establish a link between the p53 tumor suppressor and RNA processing via hnRNPA2/B1 and RNA Helicase A.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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DEAD-box RNA Helicases / metabolism*
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Heterogeneous-Nuclear Ribonucleoprotein Group A-B / metabolism*
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Humans
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Immunoprecipitation
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Neoplasm Proteins / metabolism*
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Point Mutation
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RNA / metabolism*
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RNA Processing, Post-Transcriptional
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RNA-Binding Proteins
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Tetracycline / pharmacology
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Tumor Suppressor Protein p53 / metabolism*
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Zinc Fingers
Substances
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Carrier Proteins
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Heterogeneous-Nuclear Ribonucleoprotein Group A-B
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Neoplasm Proteins
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Nuclear Proteins
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RNA-Binding Proteins
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Tumor Suppressor Protein p53
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ZMAT3 protein, human
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hnRNP A2
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RNA
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DHX9 protein, human
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DEAD-box RNA Helicases
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Tetracycline