Abstract
In the present study, we investigated the anti-inflammatory effects of a Kampo medicine Shosaikoto (TJ-9) using in vitro periodontal disease model, in which human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) from Porphyromonas gingivalis (PgLPS) produce IL-6, IL-8 and prostaglandin E2 (PGE2). Treatment with PgLPS (10 ng/ml), TJ-9 (up to 1 mg/ml) and their combinations for 24 h did not affect the viability of HGFs. Moreover, TJ-9 did not alter LPS-induced IL-6 and IL-8 productions. However, TJ-9 significantly suppressed LPS-induced PGE2 production in a dose-dependent manner but TJ-9 alone did not affect basal PGE2 level. Western blotting demonstrated that TJ-9 decreased cyclooxygenase-2 (COX-2) expression in a dose-dependent manner but not phospholipase A2. Moreover, TJ-9 selectively and dose-dependently inhibited COX-2 activity. These results suggest that TJ-9 decreased PGE2 production by inhibition of both COX-2 expression and activity and that TJ-9 may be useful to improve gingival inflammation in periodontal disease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Inflammatory Agents, Non-Steroidal*
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Blotting, Western
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Cell Survival / drug effects
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Cyclooxygenase 1 / metabolism
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Cyclooxygenase 2 / metabolism
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Dinoprostone / biosynthesis
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Dose-Response Relationship, Drug
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Drugs, Chinese Herbal / pharmacology*
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Enzyme-Linked Immunosorbent Assay
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Fibroblasts / drug effects*
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Gingiva / cytology*
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Gingiva / drug effects*
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Gingivitis / drug therapy
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Gingivitis / pathology
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Humans
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Indicators and Reagents
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Interleukin-6 / biosynthesis
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Interleukin-8 / biosynthesis
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Lipopolysaccharides / antagonists & inhibitors*
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Lipopolysaccharides / toxicity*
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Macrophages / drug effects
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Macrophages / metabolism
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Medicine, Kampo*
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Monocytes / drug effects
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Monocytes / metabolism
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Porphyromonas gingivalis / chemistry
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Tetrazolium Salts
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Thiazoles
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Drugs, Chinese Herbal
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Indicators and Reagents
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Interleukin-6
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Interleukin-8
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Lipopolysaccharides
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Tetrazolium Salts
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Thiazoles
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shosaikoto-go-keishika-shakuyakuto
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Cyclooxygenase 1
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Cyclooxygenase 2
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thiazolyl blue
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Dinoprostone