Discrimination between mammalian RNases H-1 and H-2

M Goulian; C J Heard

doi:10.1016/0003-2697(91)90555-8

Discrimination between mammalian RNases H-1 and H-2

Anal Biochem. 1991 Feb 1;192(2):398-402. doi: 10.1016/0003-2697(91)90555-8.

Authors

M Goulian¹, C J Heard

Affiliation

¹ Department of Medicine, University of California, San Diego, La Jolla 92093-0613.

PMID: 1852138
DOI: 10.1016/0003-2697(91)90555-8

Abstract

The two principal RNases H in mammalian cells, H-1 and H-2, differ in their responses to sale, divalent metal, and sulfhydryl inhibition. Specific reaction conditions that provide unambiguous discrimination between RNases H-1 and H-2 with only two assays are described. The assays were used for identification in a new purification procedure for RNases H-1 and H-2.

Publication types

Comparative Study
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Endoribonucleases / isolation & purification*
Methods
Mice
Ribonuclease H
Tumor Cells, Cultured

Substances

Endoribonucleases
Ribonuclease H

Grants and funding

CA11705/CA/NCI NIH HHS/United States