Effects of Ce3+ on conformation and activity of superoxide dismutase

Biol Trace Elem Res. 2008 Nov;125(2):170-8. doi: 10.1007/s12011-008-8165-0. Epub 2008 Jun 3.


Ce3+ in various concentrations was added to superoxide dismutase (SOD) from rat eryhrocyte in vitro to gain insight into the mechanism of molecular interactions between Ce3+ and SOD. The results showed that the reaction between SOD and Ce3 was two order, which meant that the SOD activity was markedly accelerated by a low concentration of Ce3+ and inhibited by a high concentration of Ce3+. The spectroscopic assays suggested that the Ce3+ was determined to directly bind to SOD; the binding site of Ce3+ to SOD was 0.96, and the binding constants (K(A)) were 6.78 x 10(5) and 1.68 x 10(5)L.mol(-1); the binding Ce3+ entirely altered the secondary structure of SOD. It implied that the Ce(3+) coordination created a new metal ion-active site form in SOD, thus leading to an enhancement in SOD activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cations / chemistry
  • Cations / pharmacology
  • Cattle
  • Cesium / chemistry
  • Cesium / pharmacology*
  • Circular Dichroism
  • Enzyme Activation / drug effects
  • Protein Conformation / drug effects
  • Rats
  • Spectrophotometry
  • Superoxide Dismutase / chemistry*
  • Superoxide Dismutase / metabolism*


  • Cations
  • Cesium
  • Superoxide Dismutase