Probiotic Lactobacillus acidophilus enhances the immunogenicity of an oral rotavirus vaccine in gnotobiotic pigs

Vaccine. 2008 Jul 4;26(29-30):3655-61. doi: 10.1016/j.vaccine.2008.04.070. Epub 2008 May 19.


We evaluated virus-specific B and T cell responses induced by the attenuated Wa (P1A[8]G1) human rotavirus (AttHRV) oral 2-dose vaccine with or without Lactobacillus acidophilus (LA) colonization in neonatal gnotobiotic (Gn) pigs. The AttHRV vaccinated and LA-fed pigs had a significantly higher magnitude of HRV-specific IFN-gamma producing CD8+ T cell responses in ileum and spleen, IgA and IgG antibody-secreting cell responses in ileum, and serum IgM, IgA and IgG antibody and virus neutralizing antibody titers compared to the AttHRV vaccinated pigs without LA colonization. These findings suggest that L. acidophilus has significant immunopotentiating effects and may be used as a safe oral adjuvant for rotavirus vaccines in neonates.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • B-Lymphocytes / immunology
  • Germ-Free Life
  • Humans
  • Ileum / immunology
  • Immunization, Secondary
  • Immunoglobulin A / immunology
  • Immunoglobulin G / immunology
  • Immunoglobulin M
  • Immunologic Factors / pharmacology*
  • Interferon-gamma / metabolism
  • Lactobacillus acidophilus / immunology*
  • Neutralization Tests
  • Probiotics / pharmacology*
  • Rotavirus Vaccines / administration & dosage
  • Rotavirus Vaccines / immunology*
  • Spleen / immunology
  • Swine
  • T-Lymphocytes / immunology
  • Vaccines, Attenuated / administration & dosage
  • Vaccines, Attenuated / immunology


  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Immunologic Factors
  • Rotavirus Vaccines
  • Vaccines, Attenuated
  • Interferon-gamma