Characterization of clonal relatedness among the natural population of Staphylococcus aureus strains by using spa sequence typing and the BURP (based upon repeat patterns) algorithm

J Clin Microbiol. 2008 Aug;46(8):2805-8. doi: 10.1128/JCM.00071-08. Epub 2008 Jun 4.


We evaluated the BURP (based upon repeat patterns) algorithm, which relies on sequencing of the Staphylococcus aureus protein A gene (spa), for its ability to infer clonal relatedness within a population of 110 wild-type strains. BURP clustering of the resulting 66 spa types was highly concordant with multilocus sequence typing (96.5% concordance) and pulsed-field gel electrophoresis (94.9%).

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques / methods*
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics*
  • Electrophoresis, Gel, Pulsed-Field
  • Genotype
  • Humans
  • Sequence Analysis, DNA / methods
  • Staphylococcal Protein A / genetics*
  • Staphylococcus aureus / classification*
  • Staphylococcus aureus / genetics*


  • DNA, Bacterial
  • Staphylococcal Protein A