Comparison of the growth kinetics of neuropathogenic and nonneuropathogenic equid herpesvirus type 1 (EHV-1) strains in cultured murine neuronal cells and the relevance of the D/N(752) coding change in DNA polymerase gene (ORF30)

J Vet Med Sci. 2008 May;70(5):505-11. doi: 10.1292/jvms.70.505.


We compared the growth kinetics of neuropathogenic and nonneuropathogenic equine herpesvirus type 1 (EHV-1) strains in mouse cerebral cortex cells and investigated the relevance of the D/N amino acid change at position 752 of ORF30 in Japanese isolates. Neuropathogenic electropherotype P strains 01c1 and 89c25 exhibited similar growth kinetics to nonneuropathogenic P strain 90c16 in cultured neurons; however, the growth ability of type B strain 97c7 was lower than those of the other strains tested. The amino acid encoded at 752 of ORF30 in 01c1 was asparagic acid; asparagine was encoded in the other EHV-1 strains isolated from Japanese horses. The D/N(752) difference in ORF30 may not be related to replication ability in neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • DNA-Directed DNA Polymerase / chemistry*
  • DNA-Directed DNA Polymerase / genetics
  • Gene Expression Regulation, Viral
  • Herpesvirus 1, Equid / genetics*
  • Herpesvirus 1, Equid / growth & development*
  • Herpesvirus 1, Equid / pathogenicity
  • Kinetics
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Neurons / cytology*
  • Neurons / virology*


  • DNA-Directed DNA Polymerase