The Antibody Response in Breast-Fed and Non-Breast-Fed Infants After Artificial Colonization of the Intestine With Escherichia Coli O83

Pediatr Res. 1991 Apr;29(4 Pt 1):396-9. doi: 10.1203/00006450-199104000-00013.

Abstract

The local and systemic antibody response after oral administration of a nonenteropathogenic type 1 fimbriated Escherichia coli O83 strain was followed in nine breast-fed and eight formula-fed infants during their first 15 wk of life. Five breast-fed and six formula-fed infants were followed as controls. E. coli O83 was detected in the stools of colonized infants from d 2 after colonization and persisted in the intestine for up to 26 wk. The percentage of children successfully colonized with E. coli O83 was higher among breast-fed than among formula-fed colonized infants. Also, the O83 bacteria isolated from the breast-fed children had a higher capacity to attach to colonic epithelial cells of the HT-29 cell line than those isolated from bottle-fed infants. E. coli O83 IgA and IgM antibodies estimated by ELISA were significantly elevated in the saliva of colonized as compared with control infants 2-7 wk after colonization. IgA antibodies against O83 were also higher in the stool of colonized formula-fed infants than in formula-fed controls. The results suggest that the mucosal immune system of the newborn infant can be triggered early to produce specific antibodies against bacteria colonizing the intestine.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Bacterial / biosynthesis*
  • Breast Feeding*
  • Escherichia coli / immunology*
  • Escherichia coli Infections / immunology
  • Escherichia coli Infections / prevention & control
  • Feces / microbiology
  • Female
  • Humans
  • Immunoglobulin A / biosynthesis
  • Infant
  • Infant Food
  • Infant, Newborn
  • Intestines / immunology
  • Intestines / microbiology
  • Male

Substances

  • Antibodies, Bacterial
  • Immunoglobulin A