Validation of a low cost computer-based method for quantification of immunohistochemistry-stained sections

Appl Immunohistochem Mol Morphol. 2008 Jul;16(4):400-4. doi: 10.1097/PAI.0b013e31815b5340.

Abstract

The aim of the present study was to determine if the Alpha DigiDoc RT system would be an effective method of quantifying immunohistochemical staining as compared with a manual counting method, which is considered the gold standard. Two readers were used to count 31 samples by both methods. The results obtained using the Bland-Altman for concordance deemed no statistical difference between the 2 methods. Thus, the Alpha DigiDoc RT system is an effective, low cost method to quantify immunohistochemical data.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Validation Study

MeSH terms

  • Antigens, Neoplasm / analysis
  • Antigens, Neoplasm / immunology
  • Computers* / economics
  • Humans
  • Immunohistochemistry / economics
  • Immunohistochemistry / instrumentation
  • Immunohistochemistry / methods*
  • Male
  • Prostatic Neoplasms / chemistry
  • Prostatic Neoplasms / pathology
  • Staining and Labeling / economics
  • Staining and Labeling / instrumentation
  • Staining and Labeling / methods*

Substances

  • Antigens, Neoplasm