Evaluation of microvascular endothelial function in patients with type 1 diabetes using laser-Doppler perfusion monitoring: which method to choose?

Microvasc Res. 2008 Aug;76(2):132-3. doi: 10.1016/j.mvr.2008.04.003. Epub 2008 Apr 25.


The evaluation of microvascular function is essential in the investigation of the pathophysiology of cardiometabolic diseases [Struijker-Boudier, H.A. et al., 2007. Evaluation of the microcirculation in hypertension and cardiovascular disease. Eur. Heart J. 28, 2834-2840]. In clinical research and practice, the study of microcirculation is of great value in the assessment of the effects of medical interventions and monitoring disease progression. It is well-known that patients with type 1 and 2 diabetes have microvascular dysfunction that results from numerous factors including hyperglycemia, oxidative stress and insulin resistance [Schalkwijk, C.G., Stehouwer, C.D., 2005. Vascular complications in diabetes mellitus: the role of endothelial dysfunction. Clin. Sci. (Lond). 109, 143-159]. Moreover, skin microvascular dysfunction in type 1 diabetes precedes symptoms of end-organ microvascular disease [Khan, F. et al., 2000. Impaired skin microvascular function in children, adolescents, and young adults with type 1 diabetes. Diabetes Care 23, 215-220]. In this study, we assessed skin microvascular function of patients with type 1 diabetes using laser-Doppler perfusion monitoring (LDPM) coupled with physiological and pharmacological local vasodilator stimuli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / pharmacology
  • Adult
  • Diabetes Mellitus, Type 1 / physiopathology*
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / physiopathology*
  • Female
  • Humans
  • Hyperemia / physiopathology
  • Laser-Doppler Flowmetry / methods*
  • Male
  • Microcirculation / drug effects
  • Microcirculation / physiopathology
  • Monitoring, Physiologic / methods
  • Nitroprusside / pharmacology
  • Skin / blood supply*
  • Skin / drug effects
  • Skin / physiopathology
  • Vasodilation / drug effects
  • Vasodilation / physiology


  • Nitroprusside
  • Acetylcholine