Genome organization of membrane-containing bacteriophage PRD1

Virology. 1991 Aug;183(2):658-76. doi: 10.1016/0042-6822(91)90995-n.

Abstract

We have determined the nucleotide sequence of the late region (11 kbp) of the lipid-containing bacteriophage PRD1. Gene localization was carried out by complementing nonsense phage mutants with genomic clones containing specific reading frames. The localization was confirmed by sequencing the N-termini of isolated gene products as well as sequencing the N-termini of tryptic fragments of the phage membrane-associated proteins. This, with the previously obtained sequence of the early regions, allowed us to organize most of the phage genes in the phage genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacteriophages / genetics*
  • Base Sequence
  • Cloning, Molecular
  • DNA, Viral
  • Electrophoresis, Polyacrylamide Gel
  • Genes, Viral*
  • Genetic Complementation Test
  • Gram-Negative Bacteria
  • Molecular Sequence Data
  • Mutation
  • Open Reading Frames
  • Restriction Mapping
  • Viral Envelope Proteins / genetics

Substances

  • DNA, Viral
  • Viral Envelope Proteins
  • assembly factor P17

Associated data

  • GENBANK/M55473
  • GENBANK/M55474
  • GENBANK/M55475
  • GENBANK/M55476
  • GENBANK/M55477
  • GENBANK/M55478
  • GENBANK/M64315
  • GENBANK/M64316
  • GENBANK/M69077
  • GENBANK/S42233