Abstract
We studied whether mitochondrial functions and Ca2+ metabolism were altered in Wistar Kyoto normotensive (WKY) and spontaneous hypertensive rats (SHR). Ca2+ uptake was decreased in SHR compared to WKY rats. Accumulation of Ca2+ was more efficient in WKY than in SHR rats. mDeltaPsi was lower in SHR compared to WKY rats. Basal complex IV activity was higher in SHR than WKY rats, whereas basal L-citrulline production, an indicator of nitric oxide synthesis, was decreased in SHR and dependent on Ca2+ concentration (p<0.05). Impact of Ca2+ was counteracted by EGTA. These data show an age-dependent decreased mitochondrial functions in brain mitochondria during hypertension.
Publication types
-
Comparative Study
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Aging / metabolism*
-
Animals
-
Brain / ultrastructure
-
Calcium / metabolism*
-
Calcium / pharmacology*
-
Citrulline / analysis
-
Citrulline / biosynthesis
-
Egtazic Acid / pharmacology
-
Electron Transport Complex IV / genetics
-
Electron Transport Complex IV / metabolism
-
Hypertension / metabolism*
-
Membrane Potential, Mitochondrial / drug effects
-
Mitochondria / drug effects
-
Mitochondria / enzymology
-
Mitochondria / genetics
-
Mitochondria / metabolism*
-
Nitric Oxide Synthase / metabolism
-
Oxygen Consumption / drug effects
-
Rats
-
Rats, Inbred SHR
-
Rats, Inbred WKY
-
Species Specificity
-
Spectrometry, Fluorescence
-
Time Factors
Substances
-
Citrulline
-
Egtazic Acid
-
Nitric Oxide Synthase
-
Electron Transport Complex IV
-
Calcium