HIV Type 2 Protease, Reverse Transcriptase, and Envelope Viral Variation in the PBMC and Genital Tract of ARV-naive Women in Senegal

AIDS Res Hum Retroviruses. 2008 Jun;24(6):857-64. doi: 10.1089/aid.2008.0015.

Abstract

Unique viral variants and resistance mutations may occur in the genital tract of HIV-2 ARV-naive infected women. We sequenced and phylogenetically analyzed protease (PR), reverse transcriptase (RT), and envelope (ENV) from PBMC and genital tract samples from four ARV-naive women in Senegal. HIV-2 protease polymorphisms that predict HIV-1 protease inhibitor (PI) resistance were common. Two subjects had protease mutations (T77I and I64V) in genital tract samples that were not found in PBMCs. One subject had the HIV-2 reverse transcriptase M184I mutation in CVL DNA (but not PBMCs) that is known to confer 3TC/FTC resistance in HIV-2. In another subject, the reverse transcriptase A62V mutation was also found in CVL-RNA but not PBMCs. We found no significant difference in ENV variants between PBMCs and the genital tract. HIV-2 RT and PR mutations in the genital tract of ARV-naive females may have implications for transmitted HIV-2 resistance and ARV therapy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Anti-HIV Agents / therapeutic use
  • Cervix Uteri / virology*
  • Drug Resistance, Viral / genetics
  • Female
  • HIV Infections / blood
  • HIV Infections / epidemiology*
  • HIV Protease / genetics
  • HIV Reverse Transcriptase / genetics
  • HIV-2 / genetics*
  • Humans
  • Leukocytes, Mononuclear / virology*
  • Likelihood Functions
  • Middle Aged
  • Molecular Sequence Data
  • Mutation
  • Phylogeny
  • Polymorphism, Genetic
  • RNA, Viral / analysis
  • Senegal / epidemiology
  • Sequence Analysis, Protein / statistics & numerical data
  • Vagina / virology*
  • env Gene Products, Human Immunodeficiency Virus / genetics

Substances

  • Anti-HIV Agents
  • RNA, Viral
  • env Gene Products, Human Immunodeficiency Virus
  • reverse transcriptase, Human immunodeficiency virus 2
  • HIV Reverse Transcriptase
  • HIV Protease
  • p16 protease, Human immunodeficiency virus 2