Generation and validation of affinity reagents on a proteome-wide level

J Mol Recognit. Mar-Apr 2009;22(2):57-64. doi: 10.1002/jmr.891.

Abstract

There is a need for protein-specific affinity reagents to explore the gene products encoded by the genome. Recently, systematic efforts to generate validated affinity reagents on a whole human proteome level have been initiated. There are several issues for such efforts, including choice of antigen, type of affinity reagent, and the subsequent validation of the generated protein-specific binders. The advantages and disadvantages with the different approaches are discussed and the problems related to quality assessment of antibodies to be used in multi-platform applications are addressed. This review also describes the efforts to create a virtual resource of validated antibodies using a community-based portal and summarizes the status and visions for the publicly available human protein atlas (http://www.proteinatlas.org) showing the human protein profiles in a large number of normal and cancer tissues as well as a large set of human cell lines.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Affinity Labels*
  • Humans
  • Proteins / immunology*
  • Proteins / metabolism*
  • Proteome / immunology*
  • Proteome / metabolism*

Substances

  • Affinity Labels
  • Proteins
  • Proteome