A B-Myb complex containing clathrin and filamin is required for mitotic spindle function

EMBO J. 2008 Jul 9;27(13):1852-62. doi: 10.1038/emboj.2008.118. Epub 2008 Jun 12.


B-Myb is one member of the vertebrate Myb family of transcription factors and is ubiquitously expressed. B-Myb activates transcription of a group of genes required for the G2/M cell cycle transition by forming the dREAM/Myb-MuvB-like complex, which was originally identified in Drosophila. Mutants of zebrafish B-myb and Drosophila myb exhibit defects in cell cycle progression and genome instability. Although the genome instability caused by a loss of B-Myb has been speculated to be due to abnormal cell cycle progression, the precise mechanism remains unknown. Here, we have purified a B-Myb complex containing clathrin and filamin (Myb-Clafi complex). This complex is required for normal localization of clathrin at the mitotic spindle, which was previously reported to stabilize kinetochore fibres. The Myb-Clafi complex is not tightly associated with the mitotic spindles, suggesting that this complex ferries clathrin to the mitotic spindles. Thus, identification of the Myb-Clafi complex reveals a previously unrecognized function of B-Myb that may contribute to its role in chromosome stability, possibly, tumour suppression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Clathrin / isolation & purification
  • Clathrin / metabolism*
  • Contractile Proteins / isolation & purification
  • Contractile Proteins / metabolism*
  • Fibroblasts / metabolism
  • Filamins
  • Genomic Instability
  • HeLa Cells
  • Humans
  • Mice
  • Microfilament Proteins / isolation & purification
  • Microfilament Proteins / metabolism*
  • Mitosis
  • Multiprotein Complexes / isolation & purification
  • Multiprotein Complexes / metabolism
  • Proto-Oncogene Proteins c-myb / isolation & purification
  • Proto-Oncogene Proteins c-myb / metabolism*
  • Spindle Apparatus / metabolism*


  • Clathrin
  • Contractile Proteins
  • Filamins
  • Microfilament Proteins
  • Multiprotein Complexes
  • Proto-Oncogene Proteins c-myb