Zinc finger-based knockout punches for zebrafish genes

Zebrafish. Summer 2008;5(2):121-3. doi: 10.1089/zeb.2008.9988.


The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental organism for understanding human disease. This ZFN-based technology to generate targeted knockouts in this aquatic animal opens the door to an array of new biological models of human disease and genetic testing.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Deoxyribonucleases / genetics
  • Deoxyribonucleases / metabolism
  • Gene Deletion*
  • Gene Expression Regulation / physiology
  • Zebrafish / genetics*
  • Zinc Fingers*


  • Deoxyribonucleases