Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway

Regul Pept. 2008 Nov 29;151(1-3):71-4. doi: 10.1016/j.regpep.2008.05.001. Epub 2008 May 13.


Oxytocin is a mammalian hormone that is released mainly after distension of the uterine cervix. In this study, we report that oxytocin stimulates intracellular release of calcium, and also activates AMPK (AMP-activated protein kinase) in C2C12 myoblast cells in a time/dose-dependent manner. Oxytocin receptor mRNA was detected in C2C12 cells. In addition, oxytocin stimulated glucose uptake and, moreover, inhibition of either CaMKK (Ca(2+)/calmodulin-dependent protein kinase kinase) or AMPK blocked oxytocin-mediated AMPK activation and glucose uptake. Taken together, our findings suggest that oxytocin may serve a peripheral metabolic function in skeletal muscle cells through the calcium-CaMKK-AMPK pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AMP-Activated Protein Kinases / metabolism*
  • Animals
  • Base Sequence
  • Biological Transport, Active / drug effects
  • Calcium / metabolism*
  • Calcium-Calmodulin-Dependent Protein Kinase Kinase / metabolism*
  • Cell Line
  • DNA Primers / genetics
  • Enzyme Activation / drug effects
  • Female
  • Glucose / metabolism*
  • Mice
  • Muscle Fibers, Skeletal / drug effects*
  • Muscle Fibers, Skeletal / metabolism*
  • Oxytocin / pharmacology*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Oxytocin / genetics


  • DNA Primers
  • RNA, Messenger
  • Receptors, Oxytocin
  • Oxytocin
  • Calcium-Calmodulin-Dependent Protein Kinase Kinase
  • AMP-Activated Protein Kinases
  • Glucose
  • Calcium