The pentatricopeptide repeat gene OTP51 with two LAGLIDADG motifs is required for the cis-splicing of plastid ycf3 intron 2 in Arabidopsis thaliana

Plant J. 2008 Oct;56(1):157-68. doi: 10.1111/j.1365-313X.2008.03581.x. Epub 2008 Jun 28.

Abstract

Summary The Arabidopsis thaliana chloroplast contains 20 group-II introns in its genome, and seven known splicing factors are required for the splicing of overlapping subsets of 19 of them. We describe an additional protein (OTP51) that specifically promotes the splicing of the only group-II intron for which no splicing factor has been described previously. This protein is a pentatricopeptide repeat (PPR) protein containing two LAGLIDADG motifs found in group-I intron maturases in other organisms. Amino acids thought to be important for the homing endonuclease activity of other LAGLIDADG proteins are missing in this protein, but the amino acids described to be important for maturase activity are conserved. OTP51 is absolutely required for the splicing of ycf3 intron 2, and also influences the splicing of several other group-IIa introns. Loss of OTP51 has far-reaching consequences for photosystem-I and photosystem-II assembly, and for the photosynthetic fluorescence characteristics of mutant plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Chlorophyll / metabolism
  • Chloroplasts / genetics
  • Chloroplasts / metabolism
  • DNA, Bacterial / genetics
  • DNA, Chloroplast / genetics
  • DNA, Plant / genetics
  • Genes, Plant
  • Genome, Chloroplast
  • Genome, Plant
  • Introns*
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Photosystem I Protein Complex / genetics
  • Photosystem I Protein Complex / metabolism
  • Photosystem II Protein Complex / genetics
  • Photosystem II Protein Complex / metabolism
  • Protein Interaction Domains and Motifs
  • RNA Splicing*
  • RNA, Plant / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Thylakoids / metabolism

Substances

  • Arabidopsis Proteins
  • DNA, Bacterial
  • DNA, Chloroplast
  • DNA, Plant
  • Photosystem I Protein Complex
  • Photosystem II Protein Complex
  • RNA, Plant
  • T-DNA
  • Chlorophyll