The stability of the oxidative stress marker, urinary 8-hydroxy-2'- deoxyguanosine (8-OHdG), when stored at room temperature

J Occup Health. 2008;50(4):366-72. doi: 10.1539/joh.l7144. Epub 2008 Jun 18.

Abstract

We examined the stabilities of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) stored at room temperature (25 degrees C) for 24 h or at -80 degrees C for 800 days. Subjects were 19 males and 17 females aged 23-58 yr for the 24-h study, and 9 males and 4 females aged 24-54 yr for the 800-day study. We obtained information on the subjects by questionnaires and interviews. The level of urinary 8-OHdG was measured by HPLC using two-step separations. There were no significant changes of amount of urinary 8-OHdG under either storage conditions. We conclude that urine samples can be stored at 25 degrees C and below for 24 h, when the research purpose includes the determination of urinary 8-OHdG. Urinary 8-OHdG was also stable for over two years when stored at -80 degrees C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / analysis
  • Deoxyguanosine / pharmacokinetics
  • Deoxyguanosine / urine
  • Drug Stability
  • Drug Storage*
  • Female
  • Humans
  • Interviews as Topic
  • Male
  • Middle Aged
  • Oxidative Stress*
  • Specimen Handling
  • Surveys and Questionnaires
  • Temperature*

Substances

  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine