Supplementation with pumpkin seed oil improves plasma lipid profile and cardiovascular outcomes of female non-ovariectomized and ovariectomized Sprague-Dawley rats

Phytother Res. 2008 Jul;22(7):873-7. doi: 10.1002/ptr.2381.


Pumpkin (Cucurbita species) seed oil (PSO) is a rich source of phytoestrogens and the aim of this study was to examine the effect of PSO supplementation on the total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), triglycerides, high density lipoprotein cholesterol (HDL-C), systolic and diastolic blood pressure in non-ovariectomized and ovariectomized Sprague-Dawley rats. Female rats weighing 220-300 g were divided into non-ovariectomized rats for supplementation with corn oil (control CO; n = 6) or PSO (control PSO; n = 5) and ovariectomized rats for supplementation with corn oil (OVX/CO; n = 6) or PSO (OVX/PSO; n = 5) for 5 days per week for 12 weeks (corn oil 40 mg/kg or PSO 40 mg/kg given orally). Systolic and diastolic blood pressures were measured weekly. Blood was collected at the end of the period for plasma lipid assays. Control PSO had lower TC, LDL-C, triglycerides and higher HDL-C than the control CO. The OVX/CO had higher TC, LDL-C, triglycerides and lower HDL-C than the control CO and these changes were prevented in the OVX/PSO rats. PSO supplementation also resulted in lower systolic and diastolic blood pressures in both non-ovariectomized and ovariectomized rats. It is concluded that PSO supplementation can prevent changes in plasma lipids and blood pressure associated with inadequate oestrogen availability.

MeSH terms

  • Animal Feed
  • Animals
  • Blood Pressure / drug effects
  • Blood Pressure / physiology
  • Body Weight / drug effects
  • Cholesterol, HDL / blood
  • Cholesterol, LDL / blood
  • Cucurbita / chemistry*
  • Dietary Fats / administration & dosage
  • Disease Models, Animal
  • Female
  • Hypercholesterolemia / blood
  • Hypercholesterolemia / drug therapy*
  • Hypercholesterolemia / physiopathology
  • Lipids / blood*
  • Ovariectomy*
  • Phytotherapy*
  • Plant Oils / administration & dosage*
  • Rats
  • Rats, Sprague-Dawley
  • Seeds / chemistry
  • Triglycerides / blood


  • Cholesterol, HDL
  • Cholesterol, LDL
  • Dietary Fats
  • Lipids
  • Plant Oils
  • Triglycerides