Induction of Pluripotent Stem Cells by Defined Factors Is Greatly Improved by Small-Molecule Compounds

Nat Biotechnol. 2008 Jul;26(7):795-7. doi: 10.1038/nbt1418. Epub 2008 Jun 22.

Abstract

Reprogramming of mouse and human somatic cells can be achieved by ectopic expression of transcription factors, but with low efficiencies. We report that DNA methyltransferase and histone deacetylase (HDAC) inhibitors improve reprogramming efficiency. In particular, valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than 100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent stem cells without introduction of the oncogene c-Myc.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / drug effects*
  • Cell Line
  • DNA (Cytosine-5-)-Methyltransferases / antagonists & inhibitors*
  • Histone Deacetylase Inhibitors*
  • Mice
  • Stem Cells / cytology*
  • Stem Cells / drug effects
  • Stem Cells / physiology*
  • Valproic Acid / administration & dosage*

Substances

  • Histone Deacetylase Inhibitors
  • Valproic Acid
  • DNA (Cytosine-5-)-Methyltransferases