Impaired lymphocyte responses and their restoration in oral cancer patients expressing distinct TCR variable region

Cancer Invest. 2008 Jun;26(5):471-80. doi: 10.1080/07357900701681442.

Abstract

We have analyzed TCR Vbeta gene usage and clonality of T cells in the peripheral blood of patients with oral cancer and healthy individuals. A large repertoire of clonal TCR Vbeta was observed in the peripheral blood of cancer patients, and this clonal expansion was dominantly represented in the CD8+ T cells. A marked decrease in the lymphocyte proliferative responses to mitogen and anti-CD3 MAb and spontaneous apoptosis was observed in lymphocytes. Appropriate co-stimulation of lymphocytes (anti-TCR Vbeta MAb and anti-CD28 MAb) restored the lymphocyte proliferative responses and CD3-zeta chain expression in these patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Monoclonal
  • Apoptosis
  • CD28 Antigens / blood
  • CD3 Complex / blood
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / drug effects
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / pathology
  • Case-Control Studies
  • Cell Proliferation
  • Cells, Cultured
  • Clone Cells / immunology
  • Female
  • Gene Expression Regulation, Neoplastic
  • Genotype
  • Humans
  • Lymphocyte Activation* / drug effects
  • Male
  • Middle Aged
  • Mitogens / pharmacology
  • Mouth Neoplasms / genetics
  • Mouth Neoplasms / immunology*
  • Mouth Neoplasms / pathology
  • Phenotype
  • Receptors, Antigen, T-Cell, alpha-beta / blood*
  • Receptors, Antigen, T-Cell, alpha-beta / genetics

Substances

  • Antibodies, Monoclonal
  • CD28 Antigens
  • CD3 Complex
  • CD3 antigen, zeta chain
  • Mitogens
  • Receptors, Antigen, T-Cell, alpha-beta