A suppressor of yeast spp81/ded1 mutations encodes a very similar putative ATP-dependent RNA helicase

Mol Microbiol. 1991 Apr;5(4):805-12. doi: 10.1111/j.1365-2958.1991.tb00753.x.


The spp81/ded1 mutations were isolated as suppressors of a Saccharomyces cerevisiae pre-mRNA splicing mutation, prp8-1. The SPP81/DED1 gene encodes a putative ATP-dependent RNA helicase. While attempting to clone the wild-type SPP81/DED1 gene we isolated plasmids which were able to suppress the cold-sensitive growth defect of spp81 mutants. These plasmids encoded a gene (named DBP1) which mapped to chromosome XVI and not to the SPP81/DED1 locus on chromosome XV. The cloned gene suppressed the defect of spp81/ded1 mutants when present on both high and low copy-number plasmids but complemented spp81/ded1 null mutants only when present on high copy-number plasmids. In contrast to the SPP81/DED1 gene the DBP1 gene was not essential for cell viability. The nucleotide sequence of the DBP1 gene revealed that it also encoded a putative ATP-dependent RNA helicase which showed considerable similarity at the amino acid level to the SPP81/DED1 protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Cell Survival / genetics
  • Cloning, Molecular
  • DEAD-box RNA Helicases
  • Genes, Fungal
  • Genes, Suppressor*
  • Molecular Sequence Data
  • Mutation
  • RNA Helicases
  • RNA Nucleotidyltransferases / genetics*
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*


  • Saccharomyces cerevisiae Proteins
  • Adenosine Triphosphate
  • RNA Nucleotidyltransferases
  • DBP1 protein, S cerevisiae
  • DEAD-box RNA Helicases
  • RNA Helicases

Associated data

  • GENBANK/X52371
  • GENBANK/X52372
  • GENBANK/X55993
  • GENBANK/X57178
  • GENBANK/X57179
  • GENBANK/X57180
  • GENBANK/X57181
  • GENBANK/X57182
  • GENBANK/X57183
  • GENBANK/X57184